Glycocalyx and Oxidative Stress in Endothelial Function (Part 2)

The primary objective of this project is to determine the association between endothelial glycocalyx (eGC) and myocardial revascularization in patients undergoing coronary artery bypass grafting and clinical outcomes of treatment (observational prospective study 2). Specific objective 2.1: Measure eGC thickness in coronary artery disease patients before and after surgery. Specific objective 2.2: Determine the reactivity of the bypass graft sample in in vitro experiments. Specific objective 2.3: Quantify soluble markers of eGC, oxidative stress, and inflammation from serum and PBMC samples, as well as antioxidant capacity. Specific objective 2.4: Determine the association between the results of functional tests, eGC, and markers from specific objective 2.3 with clinical outcomes of treatment.