Effect of Eliminating Red Meat Consumption on the Cardiovascular System

Biochemical and Haematological parameters: To determine blood parameters, 10 ml of peripheral blood was collected by venipuncture for serum and plasma in K3EDTA tubes. The biochemical profile was determined spectrophotometrically on a Prestige 24i chemistry analyzer (Tokyo Boeky, Tokyo, Japan) with reagents from Cormay® (Warsaw, Poland). Complete blood counts are performed on a Cell-Dyn Sapphire™ hematology analyzer (Abbott Laboratories, Santa Clara, CA, USA). Approximately 7 mL of venous blood is collected per sample in a dry gel tube and centrifuged under vacuum at 1800 g, 4°C for 10 minutes. The serum is then separated into aliquots and stored at -20°C until the desired parameters are achieved. The analytical parameters total cholesterol, HDL-C, LDL-C, triglycerides, and glucose are obtained using the corresponding kit from Cormay, Poland. Concentrations are assessed spectrophotometrically according to the protocol and using the Prestige 24i equipment (TOKYO BOEKI MEDICAL SYSTEM LTD, Tokyo, Japan). The K3EDTA tube is used to perform complete blood counts on the Cell-Dyn Sapphire™ equipment (Abbott Laboratories, Santa Clara, CA, USA). All tests were validated and subjected to quality control. Vascular parameters: Participants are placed in the supine position and have a 5-minute rest period before data collection. Subsequently, heart rate (HR), systolic blood pressure (SBP), and diastolic blood pressure (DBP) are measured. The cuff is placed on the right arm, aligned with the brachial artery, and adjusted to the arm circumference. The common carotid artery (CCA) is assessed using a GE® Vivid T8 ultrasound device with a 6-12 MHz linear probe. The participant is placed in the supine position with the neck in slight hyperextension. A longitudinal section of the CCA is taken, and the image is saved for measurement of systolic (SD) and diastolic diameters (DD) and intima-media thickness (IMT). Subsequently, peak systolic (PSV) and endodiastolic (ED) velocities of the CCA are recorded using pulsed Doppler. Assessments are performed bilaterally. All measurements are performed with the aid of an electrocardiogram electrode.