Liver injury (LI) refers to a clinical syndrome caused by a rapid decline in liver function due to various causes in a short period of time, manifested by increased transaminase and bilirubin, pain in the liver area, abdominal distension, and jaundice. Acute treatment should be systematically carried out because liver injury can lead to liver dysfunction and even liver failure, and severe liver necrosis, which is life-threatening.
Currently, peripheral blood biochemistry, ultrasound, CT, MR, and liver biopsy are routinely used in clinical practice to evaluate the physiological and pathological changes of the liver during liver injury. The assessment of liver function from plasma is indirect, and marker molecules such as transaminases are diluted in the entire blood volume. During liver failure, when various processes such as inflammatory storms occur simultaneously, the results will be disturbed. Among the above methods, liver biopsy is the gold standard. However, liver biopsy only samples 1/50,000 to 1/100,000 of the liver, resulting in significant sampling errors. This heterogeneity of the liver is particularly significant when liver disease is heterogeneous, such as during liver injury and liver failure. Therefore, it is particularly important to find a non-invasive visualization that can display liver damage and liver failure in real time.
Vitamin C (VC), also known as L-ascorbic acid, is an essential water-soluble vitamin for the human body. As part of antioxidant and carbohydrate metabolism, VC participates in the circulation between liver cells and organs, mainly involving the hexuronic acid pathway, pentose phosphate cycle, glycolysis and gluconeogenesis. Glycogenolysis, as the main source of UDP-glucuronic acid, determines the rate of the hexuronic acid pathway leading to ascorbic acid synthesis. Glycogenolysis is regulated by oxidized and reduced glutathione. Therefore, glycogen, ascorbic acid and glutathione metabolism are interrelated, and the three together affect the antioxidant status of the liver. 6-Deoxy-6-\[18F\]fluoro-L-ascorbic acid (18F-DFA) is a radioactive tracer for positron emission tomography (PET) based on the structure of natural ascorbic acid (vitamin C). Recent literature reports show that 18F-DFA PET imaging can be used to evaluate liver damage and liver failure caused by acetaminophen. It can be seen that 18F-DFA PET molecular imaging is a promising diagnostic method for acute liver function injury, which may have higher sensitivity and specificity for the diagnosis of liver function injury and the evaluation of therapeutic efficacy.
In order to further observe the clinical diagnostic effect of this imaging method, we designed this prospective exploratory clinical study to further determine the effectiveness and safety of 18F-DFA PET imaging in evaluating liver function injury. Study purpose: To evaluate the efficacy (sensitivity, specificity) of 18F-DFA PET imaging in evaluating liver function injury with clinical biochemical liver function indicators or liver puncture pathological examination as control. To evaluate the correlation between liver uptake of 18F-DFA and liver function injury indicators (clinical biochemical liver function indicators or liver puncture pathological results).
