Microfragmented Adipose Tissue Compared to Saline Injection for the Treatment of Knee Osteoarthritis

Exclusion Criteria

• •Participants who meet any of the following criteria will be excluded from participating in this study. Study eligibility of participants reporting isolated use of prohibited medications during the restricted periods defined by this protocol, will be evaluated on a case-by-case basis by the medical monitor.
• •1. Diagnosis of knee OA defined as K/L grade 1 or 4.
• •2. BMI greater than 35 kg/m2.
• •3. Diagnosis of rheumatoid arthritis, psoriatic arthritis, or any other disorder which attributes to the primary source of their knee pain, including but not limited to: osteonecrosis, radiculopathy, bursitis, tendinitis, tumor, cancer.
• •4. Presence of any clinically observed active infection including in the index knee joint, infection at the site of adipose tissue harvest, and/or any active systemic or local infection.
• •5. Undergone injection in target knee within 6 months prior to screening visit, including but not limited to corticosteroids, hyaluronic acid (HA), bone marrow concentrate (BMAC), platelet rich plasma (PRP), human cellular exosomes, amniotic fluid, or any human birth tissue.
• •6. Undergone surgical procedures of either knee within 6 months prior to the screening visit.
• •7. Bilateral knee pathology can only be treated in one knee for the study. The contralateral knee must have a WOMAC-A Visual Analog Scale (VAS) pain score of no more than 20mm at the time of screening (48-hr recall).
• •8. Index knee greater than 10 degrees varus/valgus deformities (anatomic tibiofemoral angle).
• •9. Knee pain associated with osteochondritis dissecans, ligament damage or displaced meniscus tear.
• •10. Current or historical autoimmune disease that requires immunosuppressive medication.
• •11. Any disorder affecting musculoskeletal pain and/or function, including symptomatic OA of the back, hips, or ankle that would interfere with the evaluation of the treated knee.
• •12. Planned or expected surgery within the next 12 months.
• •13. Allergy to lidocaine, epinephrine, or valium.
• •14. Diagnosis of HIV or viral hepatitis.
• •15. Use of oral systemic corticosteroids within the last 90 days and for the duration of the study.
• •16. History of any chemotherapy or radiation therapy of the targeted/treatment leg or adipose harvest site.
• •17. Active worker's compensation case.
• •18. Diagnosis of coagulation disorders (e.g., Von Willebrand's disease) and/or currently on anti-coagulant therapy.
• •19. Occurrence of knee trauma to the index knee within six months prior to screening.
• •20. Unwilling to stop usage of over-the-counter pain medication (e.g., Acetaminophen or NSAID), "Rescue Analgesics", for 7 days prior to any follow-up visit, with the exception of one "baby aspirin" per day for cardiovascular therapy or prophylaxis.
• •21. Unwilling to stop taking prescription pain or prescription anti-inflammatory medication for the duration of the study, with the exception of Tramadol during the immediate post-procedure period noted below.
• •22. Unwilling to abstain from NSAIDS for 7 days pre-injection and 2 weeks post-injection. Tramadol is allowed during the 72 hours immediately post-injection, with diary documentation of usage.
• •23. Currently taking prescription pain medication for a condition other than the index knee.
• •24. Currently in prison.
• •25. Untreated symptomatic injury of the index knee (e.g., acute traumatic injury, anterior cruciate ligament injury, clinically symptomatic meniscus injury characterized by mechanical issue such as locking or catching).
• •26. Impossibility to harvest enough adipose tissue.
• •27. Any medical issue that the clinician feels would be a contraindication to the study treatment including, but not limited to:
• •1. Uncontrolled diabetes defined as HbA1c \>7%,
• •2. History of uncontrolled hypertension defined by average systolic BP \>140 mmHg or diastolic BP \> 90 mmHg on ≥ 3 blood pressure medications,
• •3. History of cardiovascular disease,
• •4. History of cerebrovascular disease,
• •5. Uncontrolled asthma, defined as symptomatic (i.e., shortness of breath and/or wheezing) despite therapy,
• •6. History of solid organ or hematologic transplantation,
• •7. Diagnosis of non-basal cell malignancy within preceding 5 years,
• •8. Change in prescription medication within 1 month prior to enrollment,
• •9. Clinically significant abnormalities in vital signs at the time of screening defined by
• •* Systolic BP \>140 or \<90 mmHg or diastolic BP \>90 or \<60 mmHg
• •* Pulse \<55 or \>100 bpm
• •* Respiratory Rate \<9 or \>20
• •* Temperature \>99 °F
• •28. History of septic arthritis or sepsis/bacteremia in the affected knee within 6 months prior to screening, or infection requiring antibiotic treatment within the preceding 3 months.
• •29. Women who are breastfeeding.
• •30. Unwilling to use contraception for 3 months post procedure unless postmenopausal (for at least 2 years) or surgically sterilized (bilateral tubal ligation, bilateral oophorectomy, or hysterectomy).