Aqueous Biomarker Levels in Diabetic Retinopathy and Diabetic Macular Edema

The DRAGONS study is a non-interventional study investigating aqueous humor biomarker levels in patients with various stages of DR, DME and other retinal pathologies. Subjects will be included in the study if at least one eye meets any of the following criteria: Group 1 - eyes with treatment naive DR with or without DME Group 2 - eyes with DME that are incompletely responsive to anti-VEGF therapy Group 3 - eyes with various retinal conditions including eyes with DR and DME that are anti-VEGF responsive as well as non-exudative retinal pathologies such as epiretinal membrane and macular hole The study will consist of a single visit and a retrospective chart review. The study visit will consist of: 1. Screening for eligibility; 2. Aqueous humor collection; 3. Blood collection; 4. Optical coherence tomography; 5. Optical coherence tomography angiography; 6. Fundus photography; and 7. Fluorescein angiography. Approximately 50 microliters (or more, up to 150 microliters) of aqueous fluid will be collected according to standard clinical procedures. Plasma and serum will be collected according to standard clinical procedures, with citrated plasma being specifically collected in a plastic tube.