Pharmacokinetics of Verinurad and Allopurinol in Combination With Cyclosporine and Rifampicin in Healthy Volunteers

Exclusion Criteria

• •History of any clinically significant disease or disorder which, in the opinion of the Investigator, may either put the volunteer at risk because of participation in the study, or influence the results or the volunteer's ability to participate in the study.
• •Subject has a positive test result for severe acute respiratory syndrome coronavirus 2 before dosing in Treatment Period 1.
• •Has clinical signs and symptoms consistent with coronavirus disease 2019 (COVID-19) infection, eg fever, dry cough, dyspnea, sore throat, fatigue or confirmed infection by appropriate laboratory test within the last 4 weeks prior to screening or on admission.
• •History of severe COVID-19 (extracorporeal membrane oxygenation, mechanically ventilated).
• •History or presence of gastrointestinal, hepatic or renal disease, or any other condition known to interfere with absorption, distribution, metabolism, or excretion of drugs.
• •Any clinically significant illness, medical/surgical procedure, or trauma within 4 weeks prior to the first administration of verinurad.
• •Any clinically significant abnormalities in clinical chemistry, hematology, or urinalysis results, at Screening (Visit 1) and on first admission (Day -1 in Treatment Period 1) as judged by the Investigator, including:
• •Alanine aminotransferase \>1.5 × Upper limit of normal (ULN) Aspartate aminotransferase \>1.5 × ULN Bilirubin (total) \>1.5 × ULN Gamma glutamyl transpeptidase \>1.5 × ULN If any of these tests are out of range, the test can be repeated once at the Screening Visit at the discretion of the Investigator.
• •Any clinically significant abnormal findings in vital signs at Screening Visit and/or on admission (Day -1 in Treatment Period 1) to the Clinical Unit, including, but not limited to, any of the following:
• •1. Systolic blood pressure \<90 mmHg or \>140 mmHg and/or diastolic blood pressure \<50 mmHg or \>90 mmHg sustained for more than 10 minutes while resting in a supine position
• •2. Heart rate (resting, supine) \<50 or \>90 bpm
• •Any clinically significant abnormalities on 12-lead electrocardiogram at Screening Visit, as judged by the Investigator, including, but not limited to any of the following:
• •1. QTcF \> 450 ms or \< 340 ms or family history of long QT syndrome,
• •2. Any significant arrhythmia,
• •3. Conduction abnormalities,
• •4. Clinically significant PR(PQ) interval prolongation (\> 240 ms); intermittent second or third degree atrioventricular (AV) block, or AV dissociation,
• •5. Complete bundle branch block and/or QRS duration \> 120 ms.
• •Any positive result at Screening Visit for serum hepatitis B surface antigen or anti-hepatitis B core antibody, hepatitis C antibody, and human immunodeficiency virus antibody.
• •Suspicion or known Gilbert's and/or Lesch-Nyhan syndrome
• •History of hypersensitivity to drugs with a similar chemical structure or class to verinurad, allopurinol, cyclosporine or rifampicin or excipients.
• •Subjects who wear soft contact lenses (due to possible staining from rifampicin), unless the subject is prepared to refrain from wearing soft lenses throughout Treatment Period 3 until after the last PK sample collection.
• •Women of childbearing potential.
• •Carrier of the Human leukocyte antigen B\*58:01 allele.
• •Has received another new chemical or biological entity (defined as a compound which has not been approved for marketing in the US or EU) within 30 days or within 5 half-lives (whichever is longer) of the first administration of verinurad in this study.
• •Plasma donation within 1 month of screening or any blood donation/loss more than 500 mL during the 3 months prior to screening.
• •History of severe allergy/hypersensitivity or ongoing allergy/hypersensitivity, as judged by the Investigator or history of hypersensitivity to drugs with a similar chemical structure or class to Novel uric acid transporter 1 transporter inhibitor \& xanthine oxidase inhibitor.
• •Current smokers or those who have smoked or used nicotine products within the 3 months prior to screening.
• •Positive screen for drugs of abuse, cotinine or alcohol at Screening or on each admission to the Clinical Unit.
• •Use of drugs with enzyme-inducing properties such as St John's Wort within 3 weeks prior to the first administration of verinurad.
• •Use of any prescribed or non-prescribed medication including antacids, analgesics (other than paracetamol/acetaminophen), herbal remedies, megadose vitamins (intake of 20 to 600 times the recommended daily dose) and minerals during the 2 weeks prior to the first administration of IMP or longer if the medication has a long half-life.
• •Known or suspected history of alcohol or drug abuse or excessive intake of alcohol as judged by the Investigator. Excessive intake of alcohol defined as the regular consumption of more than 24 g of alcohol per day for men or 12 g of alcohol per day for women.
• •Excessive intake of caffeine-containing drinks or food (eg, coffee, tea, chocolate) as judged by the Investigator. Excessive intake of caffeine defined as the regular consumption of more than 600 mg of caffeine per day or would likely be unable to refrain from the use of caffeine-containing beverages during in-house stay at the investigational site.
• •Involvement of any AstraZeneca, Parexel or Clinical Unit employee or their close relatives.
• •Judgment by the Investigator that the subject should not participate in the study if they have any ongoing or recent (i.e., during the screening period) minor medical complaints that may interfere with the interpretation of study data or are considered unlikely to comply with study procedures, restrictions, and requirements.
• •Subjects who are vegans or have medical dietary restrictions.
• •Subjects who cannot communicate reliably with the Investigator and/or are not able to read, speak and understand the German language.
• •Vulnerable subjects, eg, kept in detention, protected adults under guardianship, trusteeship, or committed to an institution by governmental or juridical order.