1\. Aged more than 20-year-old at the time of informed consent.
2\. An unconfirmed breast tumor, or have histologically confirmed invasive breast cancer.
3\. Have radiological or objective evidence of breast tumor size ≥1 cm.
4\. Be able to comply with study procedures to collect the clinical medical information, blood sample, fresh tumor tissue, and 12 sections of paraffin embedded tumor tissue.
5\. Be able to sign an informed consent.
You may not be if
1\. Have histologically confirmed ductal carcinoma in situ (DCIS).
2\. Stage I, II or III breast cancer patients who have received neoadjuvant treatments, including chemotherapy, hormonal therapy, or targeted therapy.
3\. Stage IV or metastatic breast cancer patients who have received chemotherapy.
