Role of Circulating Innate Lymphoid Cells in Allergic Disorders

Exclusion Criteria

• •1. Female donors will be excluded if they are menopausal or have undergone total abdominal hysterectomy and/or bilateral salpingoophorectomy
• •2. For female donors, blood draw cannot occur during menstrual period time. Subjects will be excluded if they have menstrual period at timing of blood draw.
• •3. Current and past smokers.
• •4. Subjects with asthma and any other lung disease including chronic obstructive pulmonary disease, interstitial lung disease, pulmonary hypertension, sarcoidosis, cystic fibrosis.
• •5. Pregnant or lactating subjects
• •6. Subjects with hypercalcemia (\> 10.3 mg/dL), renal insufficiency (glomerular filtration rate \[GFR\] \< 50 mL/min/1.73m2), or malignancy.
• •7. Subjects with any symptoms of respiratory infection in the past 4 weeks.
• •8. Subjects unable to hold leukotriene modifiers or antihistamines for at least 24 hours prior to blood draw.
• •9. Subjects on any immunomodulators, including omalizumab, or systemic corticosteroid in the past 4 weeks.