Evaluating the Asthmatic Response to an Experimental Infection With Rhinovirus in the Atopic

Exclusion Criteria

• •Inability or unwillingness of a participant or subject's legal representative to give written informed consent and HIPPA authorization
• •Positive test for serum neutralizing antibody to RV-16. Subjects with a neutralizing antibody titer \> 1:4 will be excluded.
• •Chronic heart disease, lung diseases other than asthma, or other chronic illnesses, including primary and/or secondary immunodeficiency.
• •An upper or lower respiratory tract infection within six weeks prior to enrollment
• •Who have required nasal or sinus surgery, excluding surgery for a deviated septum within 12 months prior to enrollment.
• •Who have a 5 pack/year history of smoking, or any smoking within the last 6 months.
• •Female subjects who are or who plan to become pregnant during the study, or who are nursing a baby. Additionally, to be included in this study, a woman of child-bearing potential must have a negative urine pregnancy test at screening, during the run-in, and prior to viral inoculation and agree to use an effective method of birth control such as, but not limited to, birth control pills, contraceptive foam, diaphragm, IUD, abstinence, or condoms.
• •Absolute neutrophil count (ANC) \< 1800 cells/mm3 (or 1.8 K/uL) detected during screening within 6 weeks of enrollment.
• •Subjects with asthma
• •Criteria for exclusion will include those:
• •Who have required inhaled steroids (used for asthma), nasal steroids (used for allergic rhinitis), cromolyn, nedocromil sodium, ipratropium bromide, or leukotriene modifiers during the month prior to enrollment, oral steroids within 6 weeks of enrollment, omalizumab (Xolair®) within 12 months prior to enrollment, or who are currently using beta adrenergic blocking agents.
• •Who have been hospitalized or treated in the emergency room (unless the treatment involved the use of a bronchodilator only) for asthma within the last three years.
• •To avoid RV-16 inoculations in subjects with more restrictive lung volumes, those whose FVC is \< 80% predicted will be excluded. Subjects who are currently receiving allergen immunotherapy (IT), or who have received allergen IT within the last 3 years.
• •Subjects who have had one or more night time awakenings caused by asthma symptoms and/or who have needed their SABA (albuterol) inhaler for asthma symptoms \> 4 days during the week before enrollment, or during the week before the inoculation with RV-16.
• •Control subjects
• •Who have a positive methacholine test, or positive prick skin tests at screening under IRB protocol # 12656.