Training Effects on Skeletal Muscle Fatty Acid Metabolism

Exclusion Criteria

• •Regardless of training status, subjects must not be on medications that may affect lipid levels, specifically lipid lowering agents, birth control pills or diuretics.
• •The subjects should not be on a high fat diet (\> 45% fat) as measured by a screening questionnaire. We will also administer a PAR-Q questionnaire (attached) to establish whether the subject will be safe for exercise testing. - The female subjects must not be pregnant. A pregnancy test will be performed prior to all study visits.
• •If screening TG are \> 300 (based on 1st visit results) or fasting glucose \> 100 (based on 1st visit results), the subjects will be excluded.
• •If the subject is allergic to eggs (used in lipid emulsions), soybeans(used in lipid emulsions), or lipid emulsions the subject will be excluded from the study.
• •Subjects taking anti-platelet agents (if anti-platelet agent cannot be held for seven days) and subjects taking anticoagulation therapy will be excluded.
• •Subjects with clinically significant medical issues or a history of hematologic (platelets \< 100), hepatic (LFTs \> 2X nl), renal (Cr \> 1.5), pulmonary, or cardiac abnormalities (including abnormal EKG) will also be excluded.